10 20 30 40 | | | | | |Humanx0 ----------------AQTAAATAPRIKKFAIYRWDPDKAGDKPHMQTYKVDLNKCG-PMChicken ----------------AQTAAAATSRIKKFSIYRWDPDKPGDKPRMQTYEVDLNKCG-PMSccere --------------TATTAAATHTPRLKTFKVYRWNPDEPSAKPHLQSYQVDLNDCG-PMPcdeni MVQLTLPKNSRMRVGKTWPKPEGATNVRRFNIYRWDPDT-GENPRIDTYFVDMDKCG-PMAsperg.o. MAALRSTSRL VASSKPLFRP AVFARSYATVDAAAQDPNPSETPRTKTFHIYRWNPDQPTEKPKMQSYSLDLNKTG-PMBotrytis_c VSPSRPVESASKTSTVKEPAADSESLIKTFNIYRWNPDEPTSKPRMQSYTLDLNKTG-PM ecolix2 -------------------------MRLEFSIYRYNPDV-DDAPRMQDYTLEADEGRDMMWolinelFRD -----------------------MGRMLTIRVFKYDPQSAVSKPHFQEYKIEEAPSM--TecoliFRD ---------------------MAEMKNLKIEVVRYNPEV-DTAPHSAFYEVPYDATT--S : : :::*: *: * : Prim.cons. MVQLTLPKNSRMRV22T2222334PRI2KFSIYRWDPD22GDKPHMQTY2VDL2KCGDPM 50 60 70 80 90 100 | | | | | -- |Humanx0 VLDALIKIKNEVDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTNLNK--VSKIYPChicken VLDALIKIKNELDSTLTFRRSCREGICGSCAMNIAGGNTLACTKKIDPDLSK--TTKIYPSccere VLDALLKIKDEQDSTLTFRRSCREGICGSCAMNIGGRNTLACICKIDQNESK--QLKIYPAsperg.o. MLDALIRIKNEMDPTLTFRRSCREGICGSCAMNIDGVNTLACLCRIPTDTAK--ESRIYP Botrytis_c MLDALIRIKNEVDPTLTFRRSCREGICGSCAMNIDGVNTLACLCRIPR--DAKHETKIYP Pcdeni VLDALIKIKNEIDPTLTFRRSCREGICGSCAMNIDGGNHLACIYGMDEIKG---DVNIYPecolix2 LLDALIQLK-EKDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPWolinelFRD IFIVLNMIRETYDPDLNFDFVCRAGICGSCGMMINGRPSLACRTLTKDFEDG--VITLLPecoliFRD LLDALGYIKDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDG---MKVEA :: .* :: . *.: ** .:*** .: : . *** : .Prim.cons. VLDALIKIKNELDPTLTFRRSCREGICGSCAMNINGGNTLACITKIDDN22KGKVIKIYP 110 120 130 140 150 160 | -| | | | |Humanx0 LPHMYVIKDLVPDLSNFY-AQYKSIEPYLKKKDESQEGKQQYLQSIEEREKLDGLYECILChicken LPHMYVVKDLVPDLSNFY-AQYKSIEPYLKKKDESKQGKEQYLQSIEDRQKLDGLYECILSccere LPHMFIVKDLVPDLTNFY-QQYKSIQPYLQRSSFPKDG-TEVLQSIEDRKKLDGLYECILAsperg.o. LPHTYVVKDLVPDLTQFY-KQYKSIKPYLQRETKTEDG-LEYRQSPEERKKLDGLYECIL Botrytis_c LPHTYVVKDIVPDLTQFYKQYKS-IKPYLQHTDPAPEGKE-YLQSKEDRKKLDGLYECIL Pcdeni LPHMPVVKDLVPDLTHFY-AQHASVQPYLITETPTPDK--EWRQSIEDRKKLDGLYECVMecolix2 LPGLPVIRDLVVDMGQFY-AQYEKIKPYLLNNGQNPPAR-EHLQMPEQREKLDGLYECILWolinelFRD LPAFKLIKDLSVDTGNWFNGMSQRVESWIHAQKEHDISKLEERIEPEVAQEVFELDRCIEecoliFRD LANFPIERDLVVDMTHFI-ESLEAIKPYIIGNSRTADQG-TNIQTPAQMAKYHQFSGCIN *. : : :** * :: ::.:: . : : *: Prim.cons. LPHMPV2KDLVPDLTNFYNAQYKSIEPYL2K22E22DGK4EYLQSIEDR3KLDGLYECIL 170 180 190 200 210 220 | | | | - | |Humanx0 CACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEER-LAKLQDPFSLYRCHTIMChicken CACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDYTEER-LAQLQDPFSLYRCHTIMSccere CACCSTSCPSYWWNQEQYLGPAVLMQAYRWLIDSRDQATKTR-KAMLNNSMSLYRCHTIMAsperg.o. CACCSTSCPSYWWNSEEYLGPAILLQSYRWLADSRDEKTAER-KHALDNSMSVYRCHTIL Botrytis_c CACCSTSCPSYWWNSEEYLGPAILLQSYRWLADSRDQKKEER-KAALDNSMSLYRCHTIL Pcdeni CASCSTACPSYWWNGDRYLGPAALLHAYRWIIDSRDEATGER-LDSLEDPFKLYRCHTIMecolix2 CACCSTSCPSFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSR-LDGLSDAFSVFRCHSIMWolinelFRD CGCCIAACGTKIMR-EDFVGAAGLNRVVRFMIDPHDERTDEDYYELIGDDDGVFGCMTLLecoliFRD CGLCYAACPQFGLN-PEFIGPAAITLAHRYNEDSRDHGKKER-MAQLNSQNGVWSCTFVG *. * ::* . ::*.* : . *: *.:* . : . :: * : Prim.cons. CACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRD2AT3ERYLAQL2DPFSLYRCHTIM 220 230 240 250 260 270 | | | |Humanx0 NCTRTCPKGLNPGKAIAEIKKMMATYKEKKASV--Chicken NCTRTCPKGLNPGKAIAEIKKMMATYKEKAAAA--Sccere NCTRTCPKGLNPGLAIAEIKKSLAFA---------Asperg.o. NCSRTCPKGLNPARAIAEIKKLMAAHBotrytis_c NCSRTCPKGLNPGLAIAEIKKEMAF--------- Pcdeni NCTNTCPKGLNPAKAIASIKHMMVDRIV-------ecolix2 NCVSVCPKGLNPTRAIGHIKSMLLQRNA-------WolinelFRD ACHDVCPKNLPLQSKIAYLRRKMVSVN--------ecoliFRD YCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR * .*** : * : Prim.cons. NCTRTCPKGLNPGKAIAEIKKMMAT22EKAA33PR
10 20 30 40 | | | | | |Humanx0 ----------------
AQTAAATAPRIKKFAIYRWDPDKAGDKPHMQTYKVDLNKCG-PMChicken ----------------AQTAAAATSRIKKFSIYRWDPDKPGDKPRMQTYEVDLNKCG-PMSccere --------------TATTAAATHTPRLKTFKVYRWNPDEPSAKPHLQSYQVDLNDCG-PMPcdeni MVQLTLPKNSRMRVGKTWPKPEGATNVRRFNIYRWDPDT-GENPRIDTYFVDMDKCG-PM
Asperg.o.
MAALRSTSRL VASSKPLFRP AVFARSYATVDAAAQDPNPSETPRTKTFHIYRWNPDQPTE
KPKMQSYSLDLNKTG-PM
Botrytis_c VSPSRPVESASKTSTVKEPAADSESLIKTFNIYRWNPDEPTSKPRMQSYTLDLNKTG-PM ecolix2 -------------------------MRLEFSIYRYNPDV-DDAPRMQDYTLEADEGRDMMWolinelFRD -----------------------MGRMLTIRVFKYDPQSAVSKPHFQEYKIEEAPSM--Tecoli
Botrytis_c VSPSRPVESASKTSTVKEPAADSESLIKTFNIYRWNPDEPTSKPRMQSYTLDLNKTG-PM
Botrytis_c VSPSRPVESASKTSTVKEPAADSESLI
FRD
---------------------MAEMKNLKIEVVRYNPEV-DTAPHSAFYEVPYDATT--S : : :::*: *: * : Prim.cons. MVQLTLPKNSRMRV22T2222334PRI2KFSIYRWDPD22GDKPHMQTY2VDL2KCGDPM
50 60
70 80 90 100 | | | | | -- |Humanx0 VLDALIKIKNEVDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTNLNK--VSKIYPChicken VLDALIKIKNELDSTLTFRRSCREGICGSCAMNIAGGNTLACTKKIDPDLSK--TTKIYPSccere VLDALLKIKDEQDSTLTFRRSCREGICGSCAMNIGGRNTLACICKIDQNESK--QLKIYPAsperg.o. MLDALIRIKNEMDPTLTFRRSCREGICGSCAMNIDGVNTLACL
CRIPTDTAK--ESRIYP
Botrytis_c MLDALIRIKNEVDPTLTFRRSCREGIC
GSCAMNIDGVNTLACLCRIPR--DAKHETKIYP
Pcdeni VLDALIKIKNEIDPTLTFRRSCREGICGSCAMNIDGGNHLACIYGMDEIKG---DVNIYPecolix2 LLDALIQLK-EKDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPWolinelFRD IFIVLNMIRETYDPDLNFDFVCRAGICGSCGMMINGRPSLACRTLTKDFEDG--VITLLPecoli
LLDALGYIKDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDG---MKVEA :: .* :: . *.: ** .:*** .: : . *** : .Prim.cons. VLDALIKIKNELDPTLTFRRSCREGICGSCAMNINGGNTLACITKIDDN22KGKVIKIYP
110 120
130 140 150 160 | -| | | | |Humanx0 LPHMYVIKDLVPDLSNFY-AQYKSIEPYLKKKDESQEGKQQYLQSIEEREKLDGLYECILChicken LPHMYVVKDLVPDLSNFY-AQYKSIEPYLKKKDESKQGKEQYLQSIEDRQKLDGLYECILSccere LPHMFIVKDLVPDLTNFY-QQYKSIQPYLQRSSFPKDG-TEVLQSIEDRKKLDGLYECIL
LPHTYVVKDLVPDLTQFY-KQYKSIKPYLQRETKTEDG-LEYRQSPE
ERKKLDGLYECI
L
Botrytis_c LPHTYVVKDIVPDLTQFYKQYKS-IKP
YLQHTDPAPEGKE-YLQSKEDRKKLDGLYECIL
Pcdeni LPHMPVVKDLVPDLTHFY-AQHASVQPYLITETPTPDK--EWRQSIEDRKKLDGLYECVMecolix2 LPGLPVIRDLVVDMGQFY-AQYEKIKPYLLNNGQNPPAR-EHLQMPEQREKLDGLYECILWolinelFRD LPAFKLIKDLSVDTGNWFNGMSQRVESWIHAQKEHDISKLEERIEPEVAQEVFELDRCIEecoli
LANFPIERDLVVDMTHFI-ESLEAIKPYIIGNSRTADQG-TNIQTPAQMAKYHQFSGCIN *. : : :** * :: ::.:: . : : *: Prim.cons. LPHMPV2KDLVPDLTNFYNAQYKSIEPYL2K22E22DGK4EYLQSIEDR3KLDGLYECIL
170 180
190 200 210 220 | | | | - | |Humanx0 CACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEER-LAKLQDPFSLYRCHTIMChicken CACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDYTEER-LAQLQDPFSLYRCHTIMSccere CACCSTSCPSYWWNQEQYLGPAVLMQAYRWLIDSRDQATKTR-KAMLNNSMSLYRCHTIM
CACCSTSCPSYWWNSEEYLGPAILLQSYRWLADSRDEKTAER-KHALD
NSMSVYRCHTIL
Botrytis_c CACCSTSCPSYWWNSEEYLGPAILLQS
YRWLADSRDQKKEER-
KAALD
NSMSLYRCHTIL
Pcdeni CASCSTACPSYWWNGDRYLGPAALLHAYRWIIDSRDEATGER-LDSLEDPFKLYRCHTIMecolix2 CACCSTSCPSFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSR-LDGLSDAFSVFRCHSIMWolinelFRD CGCCIAACGTKIMR-EDFVGAAGLNRVVRFMIDPHDERTDEDYYELIGDDDGVFGCMTLLecoliFRD CGLCYAACPQFGLN-PEFIGPAAITLAHRYNEDSRDHGKKER-MAQLNSQNGVWSCTFVG *. * ::* . ::*.* : . *: *.:* . : . :: * : Prim.cons. CACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRD2AT3ERYLAQL2DPFSLYRCHTIM
220 230 240
250 260 270 | | | |Humanx0 NCTRTCPKGLNPGKAIAEIKKMMATYKEKKASV--Chicken NCTRTCPKGLNPGKAIAEIKKMMATYKEKAAAA--Sccere NCTRTCPKGLNPGLAIAEIKKSLAFA---------
NCSRTCPKGLNPARAIAEIKKLMAAH
Botrytis_c NCSRTCPKGLNPGLAIAE
IKKEMAF---------
Pcdeni NCTNTCPKGLNPAKAIASIKHMMVDRIV-------ecolix2 NCVSVCPKGLNPTRAIGHIKSMLLQRNA-------WolinelFRD ACHDVCPKNLPLQSKIAYLRRKMVSVN--------ecoli
YCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR * .*** : * : Prim.cons. NCTRTCPKGLNPGKAIAEIKKMMAT22EKAA33PR
1 Human 2 Chicken 3 Sccerev 4 Pcdeni 5 ecolix2 6 WolinelFRD 7 ecolix3 Human Chick Sccer Pcdeni ecoSD WoliFR ecoFRD 1 -- -- -- M1 -- -- -- 2 -- -- -- V2 -- -- -- 3 -- -- -- Q3 -- -- -- 4 -- -- -- L4 -- -- -- 5 -- -- -- T5 -- -- -- 6 -- -- -- L6 -- -- -- 7 -- -- -- P7 -- -- -- 8 -- -- -- K8 -- -- -- 9 -- -- -- N9 -- -- -- 10 -- -- -- S10 -- -- -- 11 -- -- -- R11 -- -- -- 12 -- -- -- M12 -- -- -- 13 -- -- -- R13 -- -- -- 14 -- -- -- V14 -- -- -- 15 -- -- T1 G15 -- -- -- 16 -- -- A2 K16 -- -- -- 17 A1 A1 T3 T17 -- -- -- 18 Q2 Q2 T4 W18 -- -- -- 19 T3 T3 A5 P19 -- -- -- 20 A4 A4 A6 K20 -- -- -- 21 A5 A5 A7 P21 -- -- -- 22 A6 A6 T8 E22 -- -- M1 23 T7 A7 H9 G23 -- -- A2 24 A8 T8 T10 A24 -- M1 E3 25 P9 S9 P11 T25 -- G2 M4 26 R10 R10 R12 N26 M1 R3 K5 27 I11 I11 L13 V27 R2 M4 N6 28 K12 K12 K14 R28 L3 L5 L7 29 K13 K13 T15 R29 E4 T6 K8 30 F14 F14 F16 F30 F5 I7 I9 31 A15 S15 K17 N31 S6 R8 E10 32 I16 I16 V18 I32 I7 V9 V11 33 Y17 Y17 Y19 Y33 Y8 F10 V12 34 R18 R18 R20 R34 R9 K11 R13 35 W19 W19 W21 W35 Y10 Y12 Y14 36 D20 D20 N22 D36 N11 D13 N15 37 P21 P21 P23 P37 P12 P14 P16 38 D22 D22 D24 D38 D13 Q15 E17 39 K23 K23 E25 T39 V14 S16 V18 40 A24 P24 P26 -- -- A17 -- 41 G25 G25 S27 G40 D15 V18 D19 42 D26 D26 A28 E41 D16 S19 T20 43 K27 K27 K29 N42 A17 K20 A21 44 P28 P28 P30 P43 P18 P21 P22 45 H29 R29 H31 R44 R19 H22 H23 46 M30 M30 L32 I45 M20 F23 S24 47 Q31 Q31 Q33 D46 Q21 Q24 A25 48 T32 T32 S34 T47 D22 E25 F26 49 Y33 Y33 Y35 Y48 Y23 Y26 Y27 50 K34 E34 Q36 F49 T24 K27 E28 51 V35 V35 V37 V50 L25 I28 V29 52 D36 D36 D38 D51 E26 E29 P30 53 L37 L37 L39 M52 A27 E30 Y31 54 N38 N38 N40 D53 D28 A31 D32 55 K39 K39 D41 K54 E29 P32 A33 56 C40 C40 C42 C55 G30 S33 T34 57 G41 G41 G43 G56 R31 M34 T35 58 -- -- -- -- D32 -- -- 59 P42 P42 P44 P57 M33 -- -- 60 M43 M43 M45 M58 M34 T35 S36 61 V44 V44 V46 V59 L35 I36 L37 62 L45 L45 L47 L60 L36 F37 L38 63 D46 D46 D48 D61 D37 I38 D39 64 A47 A47 A49 A62 A38 V39 A40 65 L48 L48 L50 L63 L39 L40 L41 66 I49 I49 L51 I64 I40 N41 G42 67 K50 K50 K52 K65 Q41 M42 Y43 68 I51 I51 I53 I66 L42 I43 I44 69 K52 K52 K54 K67 K43 R44 K45 70 N53 N53 D55 N68 -- E45 D46 71 E54 E54 E56 E69 E44 T46 N47 72 V55 L55 Q57 I70 K45 Y47 L48 73 D56 D56 D58 D71 D46 D48 A49 74 S57 S57 S59 P72 P47 P49 P50 75 T58 T58 T60 T73 S48 D50 D51 76 L59 L59 L61 L74 L49 L51 L52 77 T60 T60 T62 T75 S50 N52 S53 78 F61 F61 F63 F76 F51 F53 Y54 79 R62 R62 R64 R77 R52 D54 R55 80 R63 R63 R65 R78 R53 F55 W56 81 S64 S64 S66 S79 S54 V56 S57 82 C65 C65 C67 C80 C55 C57 C58 - Fe2S2 83 R66 R66 R68 R81 R56 R58 R59 84 E67 E67 E69 E82 E57 A59 M60 85 G68 G68 G70 G83 G58 G60 A61 86 I69 I69 I71 I84 V59 I61 I62 87 C70 C70 C72 C85 C60 C62 C63 - Fe2S2 88 G71 G71 G73 G86 G61 G63 G64 89 S72 S72 S74 S87 S62 S64 S65 90 C73 C73 C75 C88 D63 C65 C66 - Fe2S2 91 A74 A74 A76 A89 G64 G66 G67 92 M75 M75 M77 M90 L65 M67 M68 93 N76 N76 N78 N91 N66 M68 M69 94 I77 I77 I79 I92 M67 I69 V70 95 N78 A78 G80 D93 N68 N70 N71 96 G79 G79 G81 G94 G69 G71 N72 97 G80 G80 R82 G95 K70 R72 V73 98 N81 N81 N83 N96 N71 P73 P74 99 T82 T82 T84 H97 G72 S74 K75 100 L83 L83 L85 L98 L73 L75 L76 101 A84 A84 A86 A99 A74 A76 A77 102 C85 C85 C87 C100 C75 C77 C78 - Fe2S2 103 T86 T86 I88 I101 I76 R78 K79 104 R87 K87 C89 Y102 T77 T79 T80 105 R88 K88 K90 G103 P78 L80 F81 106 I89 I89 I91 M104 I79 T81 L82 107 D90 D90 D92 D105 S80 K82 R83 108 T91 P91 Q93 E106 A81 D83 D84 109 N92 D92 N94 I107 L82 F84 Y85 110 L93 L93 E95 K108 N83 E85 T86 111 N94 S94 S96 G109 Q84 D86 D87 112 K95 K95 K97 -- P85 G87 G88 113 -- -- -- -- G86 -- -- 114 -- -- -- -- K87 -- -- 115 V96 T96 Q98 D110 K88 V88 -- 116 S97 T97 L99 V111 I89 I89 M89 117 K98 K98 K100 N112 V90 T90 K90 118 I99 I99 I101 I113 I91 L91 V91 119 Y100 Y100 Y102 Y114 R92 L92 E92 120 P101 P101 P103 P115 P93 P93 A93 121 L102 L102 L104 L116 L94 L94 L94 122 P103 P103 P105 P117 P95 P95 A95 123 H104 H104 H106 H118 G96 A96 N96 124 M105 M105 M107 M119 L97 F97 F97 125 Y106 Y106 F108 P120 P98 K98 P98 Y(bp) (DOI:10.1126/science.1230593) 126 V107 V107 I109 V121 V99 L99 I99 127 I108 V108 V110 V122 I100 I100 E100 128 K109 K109 K111 K123 R101 K101 R101 129 D110 D110 D112 D124 D102 D102 D102 130 L111 L111 L113 L125 L103 L103 L103 131 V112 V112 V114 V126 V104 S104 V104 132 P113 P113 P115 P127 V105 V105 V105 133 D114 D114 D116 D128 D106 D106 D106 Plant Fdx domain 134 L115 L115 L117 L129 M107 T107 M107 Bact Fdx domain 135 S116 S116 T118 T130 G108 G108 T108 stretch 101-150 (chk) interacts with 136 N117 N117 N119 H131 Q109 N109 H109 SDHA and SDHC R21 and N-term helix 137 F118 F118 F120 F132 F110 W110 F110 138 Y119 Y119 Y121 Y133 Y111 F111 I111 139 -- -- -- -- -- N112 -- 140 A120 A120 Q122 A134 A112 G113 E112 141 Q121 Q121 Q123 Q135 Q113 M114 S113 142 Y122 Y122 Y124 H136 Y114 S115 L114 143 K123 K123 K125 A137 E115 Q116 E115 144 S124 S124 S126 S138 K116 R117 A116 145 I125 I125 I127 V139 I117 V118 I117 146 E126 E126 Q128 Q140 K118 E119 K118 147 P127 P127 P129 P141 P119 S120 P119 148 Y128 Y128 Y130 Y142 Y120 W121 Y120 149 L129 L129 L131 L143 L121 I122 I121 150 K130 K130 Q132 I144 L122 H123 I122 151 K131 K131 R133 T145 N123 A124 G123 152 K132 K132 S134 E146 N124 Q125 N124 153 D133 D133 S135 T147 G125 K126 S125 154 E134 E134 F136 P148 Q126 E127 R126 155 S135 S135 P137 T149 N127 H128 T127 156 Q136 K136 K138 P150 P128 D129 A128 157 E137 Q137 D139 D151 P129 I130 D129 158 G138 G138 G140 K152 A130 S131 Q130 159 K139 K139 -- -- R131 K132 G131 160 Q140 E140 T141 -- -- L133 -- 161 Q141 Q141 E142 E153 E132 E134 T132 162 Y142 Y142 V143 W154 H133 E135 N133 163 L143 L143 L144 R155 L134 R136 I134 164 Q144 Q144 Q145 Q156 Q135 I137 Q135 165 S145 S145 S146 S157 M136 E138 T136 166 I146 I146 I147 I158 P137 P139 P137 167 E147 E147 E148 E159 E138 E140 A138 168 E148 D148 D149 D160 Q139 V141 Q139 169 R149 R149 R150 R161 R140 A142 M140 170 E150 Q150 K151 K162 E141 Q143 A141 171 K151 K151 K152 K163 K142 E144 K142 172 L152 L152 L153 L164 L143 V145 Y143 173 D153 D153 D154 D165 D144 F146 H144 174 G154 G154 G155 G166 G145 E147 Q145 175 L155 L155 L156 L167 L146 L148 F146 176 Y156 Y156 Y157 Y168 Y147 D149 S147 177 E157 E157 E158 E169 E148 R150 G148 178 C158 C158 C159 C170 C149 C151 C149 - Fe4S4 179 I159 I159 I160 V171 I150 I152 I150 180 L160 L160 L161 M172 L151 E153 N151 181 C161 C161 C162 C173 C152 C154 C152 - Fe4S4 182 A162 A162 A163 A174 A153 G155 G153 183 C163 C163 C164 S175 C154 C156 L154 184 C164 C164 C165 C176 C155 C157 C155 - Fe4S4 185 S165 S165 S166 S177 S156 I158 Y156 186 T166 T166 T167 T178 T157 A159 A157 187 S167 S167 S168 A179 S158 A160 A158 188 C168 C168 C169 C180 C159 C161 C159 - Fe3S4 189 P169 P169 P170 P181 P160 G162 P160 - Botrytis P225F Boscalid resist 190 S170 S170 S171 S182 S161 T163 Q161 191 Y171 Y171 Y172 Y183 F162 K164 F162 192 W172 W172 W173 W184 W163 I165 G163 193 W173 W173 W174 W185 W164 M166 L164 - Putative Q ligand 194 N174 N174 N175 N186 N165 R167 N165 - Botrytis N230I Boscalid resist 195 G175 G175 Q176 G187 P166 -- -- 196 D176 D176 E177 D188 D167 E168 P166 197 K177 K177 Q178 R189 K168 D169 E167 198 Y178 Y178 Y179 Y190 F169 F170 F168 199 L179 L179 L180 L191 I170 V171 I169 200 G180 G180 G181 G192 G171 G172 G170 201 P181 P181 P182 P193 P172 A173 P171 202 A182 A182 A183 A194 A173 A174 A172 203 V183 V183 V184 A195 G174 G175 A173 204 L184 L184 L185 L196 L175 L176 I174 205 M185 M185 M186 L197 L176 N177 T175 206 Q186 Q186 Q187 H198 A177 R178 L176 207 A187 A187 A188 A199 A178 V179 A177 208 Y188 Y188 Y189 Y200 Y179 V180 H178 209 R189 R189 R190 R201 R180 R181 R179 210 W190 W190 W191 W202 F181 F182 Y180 211 M191 M191 L192 I203 L182 M183 N181 212 I192 I192 I193 I204 I183 I184 E182 213 D193 D193 D194 D205 D184 D185 D183 214 S194 S194 S195 S206 S185 P186 S184 215 R195 R195 R196 R207 R186 H187 R185 216 D196 D196 D197 D208 D187 D188 D186 217 D197 D197 Q198 E209 T188 E189 H187 218 F198 Y198 A199 A210 E189 R190 G188 219 T199 T199 T200 T211 T190 T191 K189 220 E200 E200 K201 G212 D191 D192 K190 221 E201 E201 T202 E213 S192 E193 E191 222 R202 R202 R203 R214 R193 D194 R192 223 -- -- -- -- -- Y195 -- 224 L203 L203 K204 L215 L194 Y196 M193 225 A204 A204 A205 D216 D195 E197 A194 226 K205 Q205 M206 S217 G196 L198 Q195 227 L206 L206 L207 L218 L197 I199 L196 228 Q207 Q207 N208 E219 S198 G200 N197 229 D208 D208 N209 D220 D199 D201 S198 230 P209 P209 S210 P221 A200 D202 Q199 231 F210 F210 M211 F222 F201 D203 N200 232 S211 S211 S212 K223 S202 G204 G201 233 L212 L212 L213 L224 V203 V205 V202 234 Y213 Y213 Y214 Y225 F204 F206 W203 235 R214 R214 R215 R226 R205 G207 S204 - part of Q-site?? 236 C215 C215 C216 C227 C206 C208 C205 - Fe3S4 237 H216 H216 H217 H228 H207 M209 T206 - carboxin resistance in Para deni 238 T217 T217 T218 T229 S208 T210 F207 239 I218 I218 I219 I230 I209 L211 V208 240 M219 M219 M220 M231 M210 L212 G209 241 N220 N220 N221 N232 N211 A213 Y210 242 C221 C221 C222 C233 C212 C214 C211 - Fe3S4 243 T222 T222 T223 T234 V213 H215 S212 244 R223 R223 R224 N235 S214 D216 E213 245 T224 T224 T225 T236 V215 V217 V214 246 C225 C225 C226 C237 C216 C218 C215 - Fe4S4 247 P226 P226 P227 P238 P217 P219 P216 248 K227 K227 K228 K239 K218 K220 K217 249 G228 G228 G229 G240 G219 N221 H218 250 L229 L229 L230 L241 L220 L222 V219 251 N230 N230 N231 N242 N221 P223 D220 252 P231 P231 P232 P243 P222 L224 P221 253 G232 G232 G233 A244 T223 Q225 A222 254 K233 K233 L234 K245 R224 S226 A223 255 A234 A234 A235 A246 A225 K227 A224 256 I235 I235 I236 I247 I226 I228 I225 257 A236 A236 A237 A248 G227 A229 Q226 258 E237 E237 E238 S249 H228 Y230 Q227 259 I238 I238 I239 I250 I229 L231 G228 260 K239 K239 K240 K251 K230 R232 K229 H-bonds carbonyl, Sg of C215 Fe3S4 lig ->Em+ 261 K240 K240 K241 H252 S231 R233 V230 \Cheng et al. BBA 1827 (2013) 1141–1147 262 M241 M241 S242 M253 M232 K234 E231 263 M242 M242 L243 M254 L233 M235 S232 264 A243 A243 A244 V255 L234 V236 S233 265 T244 T244 F245 D256 Q235 S237 K234 266 Y245 Y245 A246 R257 R236 V238 D235 267 K246 K246 -- I258 N237 N239 F236 268 E247 E247 -- V259 A238 -- L237 269 K248 K248 -- -- -- -- I238 270 K249 A249 -- -- -- -- A239 271 A250 A250 -- -- -- -- T240 272 S251 A251 -- -- -- -- L241 273 V252 A252 -- -- -- -- K242 274 -- -- -- -- -- -- P243 275 -- -- -- -- -- -- R244 >Human----------------AQTAAATAPRIKKFAIYRWDPDKAGDKPHMQTYKVDLNKCG-PMVLDALIKIKNEVDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTNLNK--VSKIYPLPHMYVIKDLVPDLSNFY-AQYKSIEPYLKKKDESQEGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEER-LAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATYKEKKASV-->Chicken----------------AQTAAAATSRIKKFSIYRWDPDKPGDKPRMQTYEVDLNKCG-PMVLDALIKIKNELDSTLTFRRSCREGICGSCAMNIAGGNTLACTKKIDPDLSK--TTKIYPLPHMYVVKDLVPDLSNFY-AQYKSIEPYLKKKDESKQGKEQYLQSIEDRQKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDYTEER-LAQLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATYKEKAAAA-->Sccerev--------------TATTAAATHTPRLKTFKVYRWNPDEPSAKPHLQSYQVDLNDCG-PMVLDALLKIKDEQDSTLTFRRSCREGICGSCAMNIGGRNTLACICKIDQNESK--QLKIYPLPHMFIVKDLVPDLTNFY-QQYKSIQPYLQRSSFPKDG-TEVLQSIEDRKKLDGLYECILCACCSTSCPSYWWNQEQYLGPAVLMQAYRWLIDSRDQATKTR-KAMLNNSMSLYRCHTIMNCTRTCPKGLNPGLAIAEIKKSLAFA--------->PcdeniMVQLTLPKNSRMRVGKTWPKPEGATNVRRFNIYRWDPDT-GENPRIDTYFVDMDKCG-PMVLDALIKIKNEIDPTLTFRRSCREGICGSCAMNIDGGNHLACIYGMDEIKG---DVNIYPLPHMPVVKDLVPDLTHFY-AQHASVQPYLITETPTPDK--EWRQSIEDRKKLDGLYECVMCASCSTACPSYWWNGDRYLGPAALLHAYRWIIDSRDEATGER-LDSLEDPFKLYRCHTIMNCTNTCPKGLNPAKAIASIKHMMVDRIV------->ecolix2-------------------------MRLEFSIYRYNPDV-DDAPRMQDYTLEADEGRDMMLLDALIQLK-EKDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRDLVVDMGQFY-AQYEKIKPYLLNNGQNPPAR-EHLQMPEQREKLDGLYECILCACCSTSCPSFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSR-LDGLSDAFSVFRCHSIMNCVSVCPKGLNPTRAIGHIKSMLLQRNA------->WolinelFRD-----------------------MGRMLTIRVFKYDPQSAVSKPHFQEYKIEEAPSM--TIFIVLNMIRETYDPDLNFDFVCRAGICGSCGMMINGRPSLACRTLTKDFEDG--VITLLPLPAFKLIKDLSVDTGNWFNGMSQRVESWIHAQKEHDISKLEERIEPEVAQEVFELDRCIECGCCIAACGTKIMR-EDFVGAAGLNRVVRFMIDPHDERTDEDYYELIGDDDGVFGCMTLLACHDVCPKNLPLQSKIAYLRRKMVSVN-------->ecolix3---------------------MAEMKNLKIEVVRYNPEV-DTAPHSAFYEVPYDATT--SLLDALGYIKDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDG---MKVEALANFPIERDLVVDMTHFI-ESLEAIKPYIIGNSRTADQG-TNIQTPAQMAKYHQFSGCINCGLCYAACPQFGLN-PEFIGPAAITLAHRYNEDSRDHGKKER-MAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR>Botrytis_cRSARAIFAASRPAFRTQMRTMASVDSSVPESPTVSPSRPVESASKTSTVKEPAADSESLIKTFNIYRWNPDEPTSKPRMQSYTLDLNKTGPMMLDALIRIKNEVDPTLTFRRSCREGICGSCAMNIDGVNTLACLCRIPRDAKHETKIYPLPHTYVVKDIVPDLTQFYKQYKSIKPYLQHTDPAPEGKEYLQSKEDRKKLDGLYECILCACCSTSCLSYWWNSEEYLGPAILLQSYRWLADSRDQKKEERKAALDNSMSLYRCHTILNCSRTCPKGLNPGLAIAE