Sequence alignment of c cytochromes associated with bc complexes Bos sequence: 10 20 30 40 50 | | | | | Saccharomyces MTAAEHGLHAPAYAWS-----HNGPFETFDHASIRRGYQVYR-EVCAACHSLDRVAWRTL Kluyveromyces MTAAEHGLHAPGYGWS-----HNGPLETFDHSSIRRGYQVYR-EVCAACHSLDRVAWRTM Neurospora MTPAEEGLHATKYPWV-----HEQWLKTFDHQALRRGFQVYR-EVCASCHSLSRVPYRAL Human ---SDLELHPPSYPWS-----HRGLLSSLDHTSIRRGFQVYK-QVCASCHSMDFVAYRHL Bos taurus ---SDLELHPPSYPWS-----HRGLLSSLDHTSIRRGFQVYK-QVCSSCHSMDYVAYRHL Solanum tuber. -DEAEHGLECPNYPWP-----HEGILSSYDHASIRRGHQVYQ-QVCASCHSMSLISYRDL C-ELEGANS VSASGDNVHPYALPWA-----HSGPFSSFDIASVRRGYEVYK-QVCAACHSMKFLHYRHF Euglena ----GVDSHPPALPWP-----HFQWFQGLDWRSVRRGKEVYE-QVFAPCHSLSFIKYRHF Crithidia.f. --MAGKKAHPIKRDWYWNHNDRFEIWHSLDWPSVRRGRQIYT-EVFAPCHSLGRMTFTHF Bodo caudatus --MGGKRAHPVHRDWYWEHNDHWGVWKSLDWPSVRRGRQVYA-EVFAPCHPLGKLTFMHF Ricketsia EALRPETLHLKKMKWP FDGVFGTVNREAAQRGFQVYK-EVCSVCHGLNNLYYRNL Rhodospirillum ANEGGVSLHKQDWSWK-------GIFGRYDQPQLQRGFQVFH-EVCSTCHGMKRVAYRNL Blastochloris PAQASGGDTPHLQSWS-----FAGPFGQYDKAQLRRGFQVFQ-NVCVSCHTLENGGFRNL Rb.capsulatus ALAN---SNVQDHAFS-----FEGIFGKFDQAQLRRGFQVYS-EVCSTCHGMKFVPIRTL Rb.sphaeroides ---AGG-GHVEDVPFS-----FEGPFGTFDQHQLQRGLQVYT-EVCAACHGMKFVPIRSL Paracoccus AGDSHAAAHIEDISFS-----FEGPFGKFDQHQLQRGLQVYT-EVCSACHGLRYVPLRTL B.subtilis TEAFWPN--FLLKEWM--------ITKEADIDTNAEGYKVFKEQGCISCHGDNL------ B.stearothermo TEVFWPN--FLLKEWL--------IRAEVEIDTNAEGYKIAQANTCTSCHGENL------ Hb.pylori(H2) <161>KEVFA-QSCQRCHSLDYA----- AQUIFE-C1_ YEVDEEVKAIIDNPWK---------KTESGKTIAEEGRELFI-ASCSSCHSLRYDGIYIM NEISSERIA KNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQRGAQIFT-NYCLSCHSASGMRFNRL Allochromatium -MRKLILATFLLLASGGGEHLESANIDLRDQASLQRGAKYFM-NYCTGCHSLQYMRYNRL * * * ** 60 70 80 90 100 | | | | | Saccharomyces --VGVS-HTNEEVRNMAEEFEY--DDEPDEQGNP-KKRPGKLSDYIPGPYPNEQAA Kluyveromyces --VGVS-HTNAEVRAMAEEFEY--DDEPDDQGNP-KKRPGKLADYVPGPYPNEQAA Neurospora --VGTI-LTVDEAKALAEENEY--DTEPNDQGEI-EKRPGKLSDYLPDPYKNDEAA Human --VGVC-YTEDEAKELAAEVEV-QD-GPNEDGEM-FMRPGKLFDYFPKPYPNSEAA Bos taurus --VGVC-YTEDEAKALAEEVEV-QD-GPNEDGEM-FMRPGKLSDYFPKPYPNPEAA Solanum tuber. --VGVA-YTEEETKAMAAEIEV-VD-GPNDEGEM-FTRPGKLSDRFPQPYANEAAA C.elegans --VDTI-MTEEEAKAEAADALI-ND--VDDKGAS-IQRPGMLTDKLPNPYPNKKAA Euglena --EAF--MSKEEVKNMAASFEV-DD-DPDEKGEA-RKRPGKRFDTVVQPYKNEQEA Crithidia.f. --QGF--MTREEIKQLASQYEV-IDSEPDSQGNL-NRRPGKPTDTLPTPYPNHGAA Bodo caudatus --QAF--MTREEIRKLASQYEV-IDQEPDAEGLL-LPRLGKPTDTLPAPYANQRAA Ricketsia KDIGF---SDDEIKEIAKGYTV-KD-GPNDDGEM-FERPALPYDRFVPPYPNEQAA Rhodospirillum SALG---FSEDGIKELAAEKEF-PA-GPDDNGDM-FTRPGTPADHIPSPFANDKAA Bradyrhizobium AEAGGPSYSVAQVAAFASDYKI-KD-GPNDAGDM-FERPGRPADYFPSPFPNEQAA Blastochloris PSRAAPNWPLDEVRQLAASWPV-QVKDINDKGDP-MQRAPKLPDRIPSQYANEAAA Rb.capsulatus SDDGGPQLDPTFVREYAAGLDTIID---KDSGE---ERDRKETDMFPTRVGD---- Rb.sphaeroides SEPGGPELPEDQVRAYATQFTV-TD---EETGE---DREGKPTDHFPHSALE---- Paracoccus ADEGGPQLPEDQVRAYAANFDI-TD---PETEE---DRPRVPTDHFPTVSGE---- Neisseria -KDIGLTDEEIKKNLMFTTDNV---------GDV-MHSAMNPKD------------ Bordetella -QDIGLTDQQIQDSLLFTGDKV---------GDL-MKIAMTPQD------------ Allochromatium AKDLGIDEIALRQNLLFGDAKP---------GDL-ITKAMTDDD------------ Aquifex SVAANPKWKNIEKTSGRPVYRF---------GTLYKDRFFVPKDVYEAFAHDDIQG Hb.pylori (H2) ---------------------KDKAFSDPKD------------ B.subtilis -------------------------------------------------------- B.stearothermo -------------------------------------------------------- * * 110 120 130 | | | Saccharomyces RAANQGALPPDLSLIVKAR------------------HGGCDYIFSLLTGYPDE--------- Kluyveromyces RAANQGALPPDLSLIVKAR------------------HGGPDYIFALLTGYPEE--------- Neurospora RAANNGALPPDLSLIVKAR------------------HGGCDYIFSLLTGYPDE--------- Human RAANNGALPPDLSYIVRAR------------------HGGEDYVFSLLTGYCE---------- Bos taurus RAANNGALPPDLSYIVRAR------------------HGGEDYVFSLLTGYCE---------- Solanum tuber. RFANGGAYPPDLSLITKAR------------------HNGQNYVFALLTGYRD---------- C.elegans AAANNGAAPPDLSLMALAR------------------HGGDDYVFSLLTGYLE---------- Euglena RYANNGALPPDLSVITNAR------------------HGGVDYIYALLTGYGRP--------- Crithidia.f. QFANNGAEPPDLQHAVFGK------------------EGGPDYIFSLITGYNWGNGELMEI-- Bodo caudatus QFANNGAEPPDLQTAHFGK------------------EGGSDYIFAL<eos> Ricketsia RKANNGANPPDLSLIIKAR------------------YDGANYIYSLLTSYTE---------- Rhodospirillum AAANGGAAPPDLSLLAKAR------------------PGGPNYIYSLLEGYASDSPGEPAEWW Bradyrhizobium RAANGGAAPPDLSLITKARSYGRGFPWFIFDFFTQYQEQGPDYVSAVLQGFEEK--------- Blastochloris RIIHNGAVPPDLSVIAKARTFQRGFPWWVTDIFTQYNENGVDYIVALLNGYED---------- Rb.capsulatus -----G-MGPDLSVMAKAR-AGFSGPAGSGMNQLFKGIGGPEYIYRYVTGFPEE--------- Rb.sphaeroides -------NAPDLSLMAKAR-AGFHGPMGTGISQLFNGIGGPEYIYSVLTGFPEE--------- Paracoccus -----G-MGPDLSLMAKAR-AGFHGPYGTGLSQLFNGIGGPEYIHAVLTGYDGE--------- Neisseria AAKWFGAAPPDLTLIARSK--------------------GADYLYAYMRGFYKD--------- Bordetella AKKWFGTTPPDLSVIARAK-------------SVNAGPSGADYLYTYLRTFYRD--------- Allochromatium ALKWFGVVPPDLTLVTRWR--------------------SPDWVYTYLKSFYLDDT------- Aquifex LKASLGQVPPDLSSMYLAR--------------------GEGYLYQFILNPQKV--------- Hb.pylori (H2) LANYLGSHAPDLSMMIRAK--------------------GEHGLNVFINDPQK---------- B.subtilis ---QGGAAGPSLVDSGLKP---------------------DEIKKIAV--------------- B.stearothermo ---SGGA-GPSLVGTGLTA---------------------EEIAKIAK---------------- * * * * 140 150 160 | | | Saccharomyces ------PPAGVA--LPPGSNYNPYFP------------------GGSIAMARVLFDDM-- Kluyveromyces ------PPAGVV--LPPGANYNPYFP------------------GGSIAMGRVLFDDL-- Neurospora ------PPAGAS--VGAGLNFNPYFP------------------GTGIAMARVLYDGL-- Human ------PPTGVS--LREGLYFNPYFP------------------GQAIAMAPPIYTDV-- Bos taurus ------PPTGVS--LREGLYFNPYFP------------------GQAIGMAPPIYNEV-- Solanum tuber. ------PPAGVS--IREGLHYNPYFP------------------GGAIAMPKMLNDGA-- C.elegans ------APAGVK--VDDGKAYNPYFP------------------GGIISMPQQLFDEG-- Euglena ------VPGGVQ--LSTTQWYNPYFH------------------GGIIGMPPPLTDDM-- Crithidia.f. ------PPFAPE--VKPGQFWNPYFK------------------GCVLSMPPPLSDGL-- Ricketsia ------PPAYFK--MMHGTYYNPYFP------------------GAQIAMPPPLTDGQ-- Rhodospirillum VKQQQEKGLEVA--FNEAKYFNDYFP------------------GHAISMPPPLMDDL-- Bradyrhizobium ------VPEGVT--IPEGSYYNKYFP------------------GHAIKMPKPLSDGQ-- Blastochloris ------PPERFK--VPDGSFYNKYFP------------------GHIIGMTPPIADGL-- Rb.capsulatus ------NPACAP-EGIDGYYYNEVFQVGGVPDTCKDAAGIKTTHGSWAQMPPALFDDL-- Rb.sphaeroides ------PPKCAEGHEPDGFYYNRAFQNGSVPDTCKDANGVKTTAGSWIAMPPPLMDDL-- Paracoccus ------EKEEAG----AVLYHNAAFA------------------GNWIQMAAPLSDDQ-- Neisseria PTRPSG---------WNNTVFD--------------------KVGMPHPLWEQQGV Bordetella TSKATG---------WNNLAFP--------------------NVGMPHALWDRQGP Allochromatium ------RPYG---------VNNVLFP--------------------LVGMPHVLGDLQGR Hb.pylori(H2) -----------------------LLP--------------------GTAMPRV------- Aquifex --------------------------------------------LPGTTMPQLFNPQFDP B.subtilis --------------------------------------------EGKGKMPAGVFKGN-- B.stearothermo --------------------------------------------EGQGSMPGGIFKGT-- * * * Insert between axial-ligand Met and Helix 5 in some b, g and e subdivision eubacteria: 170 | Saccharomyces VEYEDGT----------------------------------------------------- Kluyveromyces VEYEDGT----------------------------------------------------- Neurospora VDYEDGT----------------------------------------------------- Human LEFDDGT----------------------------------------------------- Bos taurus LEFDDGT----------------------------------------------------- Solanum tuber. VEYEDGI----------------------------------------------------- C.elegans IEYKDGT----------------------------------------------------- Euglena IEYEDGT----------------------------------------------------- Crithidia.f. VDYEDGT----------------------------------------------------- Ricketsia VTYMDGT----------------------------------------------------- Rhodospirillum ITYEDGT----------------------------------------------------- Bradyrhizobium VTYDDGS----------------------------------------------------- Blastochloris VTYGDGT----------------------------------------------------- Rb.capsulatus VTYEDGT----------------------------------------------------- Rb.sphaeroides VEYADGH----------------------------------------------------- Paracoccus VTYEDGT----------------------------------------------------- Neisseria QAVELDAKGQPVMVK------------DEHGEMKPKLY-----WESTGLHSRRLPNGKVI Bordetella RELTTVAVHQVEAADGTRNWERVTTVYDAQGYATAKTEPLADYRGHGSLETRFKAVNPAQ Allochromatium QEAVMEPSHEP------------------GGEPTIKG-------------VKLVEEGGLS Hb.pylori(H2) -GLNEKAQKQVISYL------------EKAGDRKKHER---------------------- Aquifex ------------------------------------------------------------ B.subtilis -------------DKQLEELAKFISETTAKGN---------------------------- B.stearothermo -------------D---EELQKMAN-SSPAGT---------------------------- 180 190 200 210 220 230 240 | | | | | | | Saccharomyces PATTSQMAKDVTTFLNWCAEPEHDERKRLGLKTVIILSSLYLLSIWVKKF---KWAGIKTRKFVFNPPK Kluyveromyces PATTSQMAKDVTTFLHWCSEPEHDERKRLGLKAMIVLSSLYLLSVWVKKF---KWASIKSRKIVFNPPK Neurospora PASTSQMAKDVVEFLNWAAEPEMDDRKRMGMKVLVVTSVLFALSVYVKRY---KWAWLKSRKIVYDPPK Human PATMSQIAKDVCTFLRWASEPEHDHRKRMGLKMLMMMALLVPLVYTIKRH---KWSVLKSRKLAYRPPK Bos taurus PATMSQVAKDVCTFLRWAAEPEHDHRKRMGLKMLLMMGLLLPLVYAMKRH---KWSVLKSRKLAYRPPK Solanum tuber. PATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRL---RWSVLKSRKLVLDVVN C.elegans PATMSQQAKDVSAFMHWAAEPFHDTRKKWALKIAALIPFVAVVLIYGKRH---IWSFTKSQKFLFKTVK Euglena PASVPQMAKDVTCFLEWCSNPWWDERKLLGYKTIATLAVIAVSSGYYNRFLSGLWRSRRLAFRPFNYSK Crithidia.f. PATISQMAKDV Ricketsia NASVEQMSKDVTVFLQWAAEPEMEHRKSMGLKVMMFLIVFTILFYIAKNR---IWSNVK---------- Rhodospirillum AATKDQMAQDVVAYLNWAAEPELDARKSLGLKVLLFLGVLTAMLLALKLA---IWRDVKH--------- Bradyrhizobium PATVAQYSKDVTTFLMWTAEPHMEARKRLGFQVFVFLIIFAGLMYFTKKK---VWADSH---------- Blastochloris PETQLQYSKDVAAFLMWAAEPTLDVRKRIGWWVLGFLVIFTGLLVATKIV---VWRPVKKGLA------ Rb.capsulatus PATVDQMGQDVASFLMWAAEPKLVARKQMGLVAVVMLGLLSVMLYLTNKR---LWAPYKRQKA------ Rb.sphaeroides DASVHAMAEDVSAFLMWAAEPKLMARKQAGFTAVMFLTVLSVLLYLTNKR---LWAGVKGKKKTNV--- Paracoccus PATVDQMATDVAAFLMWTAEPKMMDRKQVGFVSVIFLIVLAALLYLTNKK---LWQPIKHPRKPE---- Neisseria QKEYDAYVRDLVNYLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKK--EYWKDVH---------- Bordetella SADYDQDVADLTAFMSWMAEPVQLYRKQLGVWVLLFLG-LFLVVAWRLNA--AYWKHVR---------- Allochromatium PQEYDTMVRDITNFLTYAGEYFQLERERIGRYVLLFLG-FLFILAYLLKK--EYWKDVH---------- Hb.pylori(H2) ----------------------------------NTLGIKIMIFFAVLSFLAYAWKRKVWSEVH----- Aquifex -QAKEKVAKIVAYMKSVNTPPPKESAKRT------VMGVIVIAYFIVMGL--LLWKYRENLLKRLGYH- B.subtilis DKQLEEL-AKFISETTAK--------------------------------------------------- B.stearothermo D---EEL-QKMANSSPA---------------------------------------------------- * * C-term Extensions in some eukaryotes*: Saccharomyces PRK---------- Kluyveromyces --K---------- Neurospora SPPPATNLALPQQ Human ------------- Bos taurus ------------- Solanum tuber. ------------- C.elegans GREPPKAQ----- * While yeast are not multicellular, many fungi are, so fungi are included in the group currently labeled fungi/metazoa, which we abbreviate "metazoa". Actually we mean to include plants, so "Eukaryote crown group"