50 60
70 80 -
90 100
| |
| | *-
| * |
Rhdbactersphaer. TGIVLAMHYTPHVDLAFASVEHIMRNVNGGFMLRYLH-ANGASLFFIAVYLHIFRGLYYGS
Paracoccus TGIVLVMHYTPHVDLAFASVEHIMRDVNGGYMLRYLH-ANGASLFFLAVYIHIFRGLYYGS
Rickettsiap TGVILAMHYTPHVDHAFESVERIMRNVNYGWLLRYTH-AVGASMFFAAIYLHIARGLYYGS
Bostaurus TGLFLAMHYTSDTTTAFSSVTHICRDVNYGWIIRYMH-ANGASMFFICLYMHVGRGLYYGS
chicken
TGLLLAMHYTADTSLAFSSVAHTCRNVQYGWLIRNLH-ANGASFFFICIFLHIGRGLYYGS
cichlasoma_boliv TGLFLAMHYTSDIATAFSSVAHICRDVNYGWLIRNMH-ANGASFFFICIYLHIGRGLYYGS
Saccharocere TGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLH-ANGASFFFMVMFMHMAKGLYYGS
Soltuber
TGVFLAMHYTPHVDLAFNSVEHIMRDVEGGWLLRYMH-ANGASMFFIVVYLHIFRGLYYAS
Polytomella TGILLAMHYCPDTSLAFYSVIHLTVDVPYGFVIRYFH-MNGASLFFVAVFLHLFRNLYYNS
Chromatiumvinosum TGVWLAMSYKPDAGLAFASVEYIMRDVDWGWLIRYMH-STGASMFFIVIYLHMFRGLLRGS
Aquifexa
SGMVLILYYNLQSQTLL-TVHLLDNGRNSLRMALQAHTRTGANFFMAIVYLHMFTGIYYNA
Chlorobiumlimicol TGLLLLQYYKPTETDAFASFLFIQGEVPFGWLLRQIH-AWSANLMIMMLFIHMFSTFFMKS
B.stearothp SGMFLTMYYVPDIKNAWESVYYLQNEVAFGQIVRGMH-HWGASLVIVMMFLHTVRVFFQGA
Synechpcc TGFAMTFYYKPTVTEAFASVQYIMNEVNFGWLIRSIH-RWSASMMVLMMILHVFRVYLTGG
Spinacia
TGFAMTFYYRPTVTDAFASVQYIMTEVNFGWLIRSVH-RWSASMMVLMMILHVFRVYLTGG
Chlamyrb6 TGFAMTFYYRPTVAEAFASVQYIMTDVNFGWLIRSIH-RWSASMMVLMMVLHVFRVYLTGG
**. :.: * . ** ** : :*. *:::* :*
.**::.: : :*: : ..
Chlorobiumlimicola
------------------------------------
110 120
130 140
150 ---------- 160
| |
| |
| ---------- |
Rhdbactersphaer. YKAPREVTWIVGMLIYLAMMATAFMGYVLPWGQMSFWGATVITGLFG----------AIPGIGHSIQTWL
Paracoccus YKAPREVTWIVGMLIYLMMMGTAFMGYVLPWGQMSFWGATVITGLFG----------AIPGVGEAIQTWL
Rickettsiap YKTPRELLWHIGIIIFLIMMATAFMGYVLPWGQMSYWGATVITNLFS----------AIPLVGEPIVIWL
Bostaurus YT--FLETWNIGVILLLTVMATAFMGYVLPWGQMSFWGATVITNLLS----------AIPYIGTNLVEWI
chicken
YL--YKETWNTGVILLLTLMATAFVGYVLPWGQMSFWGATVITNLFS----------AIPYIGHTLVEWA
cichlasoma_boliv YL--YKETWNVGVVLLLLVMMTAFVGYVLPWGQMSFWGATVITNLLS----------AVPYIGNSLVQWL
Saccharocere YRSPRVTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGATVITNLFS----------AIPFVGNDIVSWL
Soltuber
YSSPREFVRCLGVVIFLLMIVTAFIGYVLPWGQMSFWGATVITSLAS----------AIPVVGDTIVTWL
Polytomella GSQPRELLYISGVVILLLMVITAFIGYVLPWGQMSFWGATVITSLVS----------AVPIVGTDLVYYL
Chromatiumvinosum YRKPRELLWMIGVVIYLVMMATAFFGYLLPWGQMSYWGAQVIVNLFA----------AVPVVGEDLSVWV
Aquifexa
YKRPRELVWIVGWLIYFVLILTALSGYLLPWGQLSYWGFIVTTEIPGSLADAPILKPIFKAIAETIVLWM
Chlorobiumlimicol YRKPRELMWVSGFVLLLLSLGFGFTGYLLPWNELAFFATQVGTEVPK----------VAPG-GAFLVEIL
B.stearothp YKKPREMNWIVGVLIFMVMMGLGFTGYLLPWDMKALFATKVGLQIVE----------AVPLIGPAIKTLL
Synechpcc FKKPRELTWVVGVMLAVTTVTFGVTGYSLPWDQVGYWAVKIVSGVPA----------AIPVVGDQLVTLM
Spinacia
FKKPRELTWVTGVVLGVLTASFGVTGYSLPWDQIGYWAVKIVTGVPD----------AIPVIGSPLVELL
Chlamyrb6 FKRPRELTWVTGVIMAVCTVSFGVTGYSLPWDQVGYWAVKIVTGVPD----------AIPGVGGFIVELL
: . *::: :
.. ** :.*:..*.. :: .: *:* :* : DCCD-Binding
170 - 180 -
190 200
210
| - | -
| |
| |
Rhdbactersphaer. LGGPAVDNA-TLNRFFSLH-YLLPFVIAALVAIHIWAFHSTGNNNPTGVEVRRTSK-----
Paracoccus LGGPAVDNP-TLNRFFSLH-YLLPFVIAALVVVHIWAFHTTGNNNPTGVEVRRGSK-----
Rickettsiap WGGFSVDNP-TLNRFFALH-YLFPFIIVVLVILHLVALHQHGSNNPKGIDVK---------
Bostaurus WGGFSVDKA-TLTRFFAFH-FILPFIIMAIAMVHLLFLHETGSNNPTGIS-----------
chicken
WGGFSVDNP-TLTRFFALH-FLLPFAIAGITIIHLTFLHESGSNNPLGIS-----------
cichlasoma_boliv WGGFSVDNA-TLTRFFAFH-FLLPFIIAAMTMIHLIFLHETGSTNPAGLN-------------
Saccharocere WGGFSVSNP-TIQRFFALH-YLVPFIIAAMVIMHLMALHIHGSSNPLGIT-----------
Soltuber
WGGFSVDNA-TLNRFFSLH-YLLPFILVGASLLHLAALHQYGSNNPLGVH-----------
Polytomella WGGFSVSNP-TLNRFFSFH-YLLPFVLAGLSIAHLAALHSYGSTNPLSIN-----------
Chromatiumvinosum RGDFVISDA-TLNRFFAFH-FLLPFLLAGLVFLHIVALHHVGSNNPDGIEIKEGPKGNRWS
Aquifexa
KGGYVVTDV-TLGRVFGSHVLIYPLILLALVGIHLYLVRAAGISNPEGIEYDKKK------
Chlorobiumlimicol RGGPEVGGE-TLTRMFSLHVVLLPGLVMLVLAAHLTLVQILGTSAPIGYKEAG-----LIK
B.stearothp AGDPEIVGAQTLARFFAIHVFFLPAALLGLMAAHFLMIRRQGISGPLMHRGKGMKFVGDSR
Synechpcc RGSESVGQA-TLTRFYSLHTFVLPWAIAVLLLLHFLMIRKQGISGPLMSIMKKPDLSDP-K
Spinacia
RGSASVGQS-TLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPLMGVTKKPDLNDP-V
Chlamyrb6 RGGVGVGQA-TLTRFYSLHTFVLPLLTAVFMLMHFLMIRKQGISGPL
*. :...*: **:::* .::* :*:
:: * ..*
220 230
240 ------250
260
| |
| ------ |
|
Rhdbactersphaer. AEAQKDT-VPFWPYFIIKDVFALAVVLLVFFAIVGFMP------NYLGHPDNYIEANPLSTPA
Paracoccus EEAKKDT-LPFWPYFVIKDLFALAVVLVVFFAIVGFMP------NYLGHPDNYIEANPLVTPA
Rickettsiap --STKDT-IPFHPYYTVKDFVGFGVYFIIFAYFIFYAP------NYLGHPDNYIPANPLVTPA
Bostaurus --SDVDK-IPFHPYYTIKDILGALLLILALMLLVLFAP------DLLGDPDNYTPANPLNTPP
chicken
--SDSDK-IPFHPYYSFKDILGLTLMLTPFLTLALFSP------NLLGDPENFTPANPLVTPP
cichlasoma_boliv --SDAEK-ISFHPYFSYKDLLGFAVLLVALIALALFSP------NLLGDPDNFIPANPLVTPP
Saccharocere --GNLDR-IPMHSYFIFKDLVTVFLFMLILALFVFYSP------NTLGQPDNYIPGNPLVTPA
Soltuber
--SEMDK-IASYPYFYVKDLVGWVAFAIFFSIWIFYAP------NVLGHPDNYIPANPMSTPP
Polytomella ---SVAK-VPFGSYYIIKDLLGVLIIGAAMIVLAFFFP------EALNHSDNYIPANPYSTPA
Chromatiumvinosum DKAPADG-IPFHPYYTVKDLMGVVVFLAIFGYVMFFNP---TMGGLFLEAPNFQPANPMQTPA
Aquifexa
---NPDKFVPFHPYMTLKEGAYVMWYLAVFFFFVFFHI------SHFLPPENFEPANPLKTPA
Chlorobiumlimicola GYDKFFPTFLAKDGIGWLIGFALLIYLAVMFP------WEIGVKANPLSPAPL
B.stearothp IPAVRKPNIPKDYSEYPGKTEVFWPNFLLKEWLVGSVFLVG-FLCLTVAHPSPLERIADPTDTTY
B.stearothp?
GDSRIPAVRKPNIPKDYSEYPGKTEVFWPNFLLKEWLVGSVFLVGFLCLTVAHPSPLERIADPTDTTY
Synechpcc LRAKLAQ-NMGHNYYG--EPAWPNDILFTFPICIAGTIGLITGLAILDPAMIGEPGNPFATPL
Spinacia
LRAKLAK-GMGHNYYG--EPAWPNDLLYIFPVVILGTIACNVGLAVLEPSMIGEPADPFATPL
. *:
.
. .:*: **
270 280
290 300
| | ------------------
| |
Rhdbactersphaer. HIVPEWYFLPFYAILRAFTADVWVVQIANFISFGIIDAKFFGVLAMFGAILVMAL-VPWLD
Paracoccus HIVPEWYFLPFYAILRAFTADVWVVMLVNWLSFGIIDAKFFGVIAMFGAILVMAL-VPWLD
Rickettsiap HIVPEWYFLPFYAILRAV------------------PSKLGGVFLMFGSIVVLFL-LPWLD
Bostaurus HIKPEWYFLFAYAILRSI------------------PNKLGGVLALAFSILILAL-IPLLH
chicken
HIKPEWYFLFAYAILRSI------------------PNKLGGVLALAASVLILFL-IPFLH
cichlasoma_boliv HIKPEWYFLFAYAILRSI------------------PNKLGGVLALLFSILILML-VPILH
Saccharocere SIDPEWYLLPFYAILRSI------------------PDKLLGVITMFAAILVLLV-LPFTD
Soltuber
HIVPEWYFLPIYAILRSI------------------PDKVGGVAAIALVFICLLA-LPFFK
Polytomella HIVPEWYFLPVYAILRSI------------------PDKGIGILAVLLFFIGLGL-QPFLS
Chromatiumvinosum HIAPVWYFTPFYAMLRAV--------------PPMYGSQFPGVVVMFAAILILFV-LPWLD
Aquifexa
HIAPEWYLLGYYEVFRSI------------------PSKFWGFVAFNALLLLLLL-LPFLD
Chlorobiumlimicol GIKPEWYFWAQFQLLK--------------------DFKFEGGELLAIILFTIGG-VVWLL
B.st.
IPLPDWYFLFLYQLLKYSY--------------ASGPYTVIGAIVMPGLAFGALLLAPFLD
Synechpcc EILPEWYLYPVFQILRVL------------------PNKLLGIACQGAIPLGLMM-VPFIE
Spinacia
EILPEWYFFPVFQILRTV------------------PNKLLGVLLMASVPAGLLT-VPFLE
* * **: . :**
:. *: : :*
.
310 320
330 340
350 360
| -|
| |
| |
Rhdbactersphaer. TSPVRSGR-YRPMFKIYFWLLAADFVILTWVGAQQTTFPYDWISLIASAYWFAYFLVILP
Paracoccus TSRVRSGQ-YRPLFKWWFWLLAVDFVVLMWVGAMPAEGIYPYIALAGSAYWFAYFLIILP
Rickettsiap TSKIRSGN-YRPIYRIAFWIFMADCLFLGYLGSKPVSEPYITISRFAVCYYFCHFLLVLP
Bostaurus TSKQRSMM-FRPLSQCLFWALVADLLTLTWIGGQPVEHPYITIGQLASVLYFLLILVLMP
chicken
KSKQRTMT-FRPLSQTLFWLLVANLLILTWIGSQPVEHPFIIIGQMASLSYFTILLILFP
cichlasoma_boliv TSKLRALT-FRPLTQCLFWLLVADVMILTWIGGMPVEHPFIIIGQIASILYFTIFLILLP
Saccharocere RSVVRGNT-FKVLSKFFFFIFVFNFVLLGQIGACHVEVPYVLMGQIATFIYFAYFLIIVP
Soltuber
SMYVRSSS-FRPIYQGMFWLLLADCLLLGWIGCQPVEAPFVTIGQISSLVFFLFFAI-TP
Polytomella QGKA------EPHFYASFI---GGLFVLGWLGSKEITDVTSAAPSFFTLFTFAYLFVVSP
Chromatiumvinosum RSPVKSMRYKGPIFKWATGIFVVSFVALAWLGIQPASDFYTLLSQIFTVLYFAYFLL-MP
Aquifexa
FSPLKSAR-RRPLFFVMFVIFMISSMALTILGTMPPTPQNAKLGLIFAALVFAFFIS-LP
Chlorobiumlimicol VPFIDRQASEEKKSPIFTIFGILVLAFLLINTYRVYAEYSMLK----------------
B.st.
RGPE-RRPWKRPVATGMMLLTLAAIVYLTWESVVTHDWEKAAEQGKIRAEVEIDTN
Synechpcc SVNKFQNPFRRPVAMAVF--LFGTAVTL-WLGAGACFPIDESLTLGLF------------
Spinacia NVNKFQNPFRRPVATTVF--LVGTVVAL-WLGIGATLPIDKSLTLGLF------------
:: :
: * .
370 380
| |
Rhdbactersphaer. ILGAIEKPVAPPATIEEDFNAHYSPATGGTKTVVAE
Paracoccus LLGIIEKPDAMPQTIEEDFNAHYGPETHPAE-----
Rickettsiap LIGKYEKPLPLPKVL---------------------
Bostaurus TAGTIENKLLKW------------------------
chicken
TIGTLENKMLNY------------------------
cichlasoma_boliv TAGLTENKI---------------------------
Saccharocere VISTIENVLFYIGRVNK-------------------
Soltuber
ILGRVGRGIPNSYTDETDHT----------------
Polytomella LYSLLYNRVKG-------------------------
Chromatiumvinosum IYSSLDKTKPFLRG----------------------
Aquifexa
IISFIEYGWYKAKGGQQE------------------
B.st. 181 aegykiaqantCTSCHgenlsggagpslvgtgltaeeiakiakegqgsmpggifkgtdee
Synechpcc ------------------------------------
Spinacia
------------------------------------
Bacillus st.:N-term is suIV, C-term is cyt c1
1 MHRGKGMKFV GDSRIPAVRK PNIPKDYSEY PGKTEVFWPN FLLKEWLVGS VFLVGFLCLT
61 VAHPSPLERI ADPTDTTYIP LPDWYFLFLY QLLKYSYASG PYTVIGAIVM PGLAFGALLL
121 APFLDRGPER RPWKRPVATG MMLLTLAAIV YLTWESVVTH DWEKAAEQGK IRAEVeidtn
181 aegykiaqan tCTSCHgenl sggagpslvg tgltaeeiak iakegqgsmp ggifkgtdee
241 lqkmansspa
Note suIV ends w helix G
, so C-term on periplasmic side where cyt c1 N-term is.
BC1-LIKE VS B6F LIKE:
Split cyt b
15 residues between H ligands in helix D
H202 - R
C at start of helix A