Rieske Iron-sulfur Protein: Structure-based Sequence alignment
0
10
20
30 40
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Bostaurus
SHTDIKVPDFSDYRRPEVLDSTKSSKESSEARKGFSYLVTATTTVGV
Chicken
VHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATACVAT
Sachcere
-KSTYRTPNFDDVLK---------ENNDADKGRSYAYFMVGAMGLLS
Rbsphaer
---------------------MSNAEDHAGTRRDFLYYATAGAGAVA
R
bcapsul ---------------------MSHAEDNAGTRRDFLYHATAATGVVV
HHHHHHHHHHHHHHHHHH
50
60
70
80
90 100
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Bostaurus
AYAAKNVVSQFVSSMSASADVLAMSKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTKKEID
Chicken
AYAAKNVVTQFISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEIN
Sachcere
SAGAKSTVETFISSMTATADVLAMAKVEVNLAAIPLGKNVVVKWQGKPVFIRHRTPHEIQ
Rbsphaer TGAA---VWPLINQMNPSADVQALASIFVDVSSVEPGVQLTVKFLGKPIFIRRRTEADIE
Rbcapsul
TGAA---VWPLINQMNASADVKAMASIFVDVSAVEVGTQLTVKWRGKPVFIRRRDEKDIE
HHH-TMH-HHH
HHHH --b1-- --b2--- ---b3--- -HELIX-
110
120
130 140
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Bostaurus
QEAAVEVSQLRDP------------QHDLERVK-------KPEWVILIGVCTHLGCVPIAN
Chicken
QEAEVDVSKLRDP------------QHDLDRVK-------KPEWVILVGVCTHLGCVPIAN
Sachcere
EANSVDMSALKDP------------QTDADRVK-------DPQWLIMLGICTHLGCVPIGE
Rbsphaer LGRSVQLGQLVDTNARNANIDAGAEATDQNRTLD-----EAGEWLVMWGVCTHLGCVPIGG
Rbcapsul
LARSVPLGALRDTSAENANK-PGAEATDENRTLPAFDGTNTGEWLVMLGVCTHLGCVPMGD
-HELIX- turn 3-10 ---b4-- -b5
150
160
170
180
190 200
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Bostaurus
-AGDFGGYYCPCHGSHYDASGRIRKGPAPLNLEVPSYEFTSDDMVIVG
Chicken
-SGDFGGYYCPCHGSHYDASGRIRKGPAPYNLEVPTYQFVGDDLVVVG
Sachcere
-AGDFGGWFCPCHGSHYDISGRIRKGPAPLNLEIPAYEFDGD-KVIVG
Rbsphaer VSGDFGGWFCPCHGSHYDSAGRIRKGPAPENLPIPLAKFIDETTIQLG
Rbcapsul
KSGDFGGWFCPCHGSHYDSAGRIRKGPAPRNLDIPVAAFVDETTIKLG
-b6- -b7-
-b8-- --b9- ------
Underlined region 137-181 is defined by Iwata et al. as the
cluster-binding fold.
Pro175 may be cis in int position.
3-10 helix centered about114 (just after long helix) and 124
loops before and after the 124 helix are longer in Rb.
comparing yeast 1ezv and beef 1be3- towards N-term
Start to diverge by 36, quite far appart but alignable at 32, then
completely
different till ~15 (y 44)
Then close to 6 (yeast 35)
E1 to E11 are extended like beta, and lying over the multisubunit beta strand involving D,G,A
Vertical
alignment with all sequences numbered,
for
quickly finding corresponding residue number
in
different species:
BOS CHICK Yeast
Y.Pre Rb.s Rb.c
30 S1
V1 --
X30 --
--
31 H2
H2 K1
K31 --
--
32 T3
N3 S2
S32 --
--
33 D4
D4 T3
T33 --
--
34 I5
V5 Y4
Y34 --
--
35 K6
T6 R5
R35 --
--
36 V7
V7 T6
T36 --
--
37 P8
P8 P7
P37 --
--
38 D9
D9 N8
N38 --
--
39 F10
F10 F9
F39 --
--
40 S11
S11 D10
D40 --
--
41 D12
A12 D11
D41 --
--
42 Y13
Y13 V12
V42 --
--
43 R14
R14 L13
L43 --
--
44 R15
R15 K14
K44 --
--
45 P16
E16 --
-- --
--
46 E17
D17 --
-- --
--
47 V18
V18 --
-- --
--
48 L19
M19 --
-- --
--
49 D20
D20 --
-- --
--
BOS CHICK Yeast
Y.Pre Rb.s Rb.c
50 S21
A21 --
-- --
--
51 T22
T22 --
-- M1
M1
52 K23
T23 --
-- S2
S2
53 S24
S24 --
-- N3
H3
54 S25
S25 E15
E45 A4
A4
55 K26
Q26 N16
N46 E5
E5
56 E27
T27 N17
N47 D6
D6
57 S28
S28 D18
D48 H7
N7
58 S29
S29 A19
A49 A8
A8
59 E30
E30 D20
D50 G9
G9
60 A31
D31 K21
K51 T10
T10
61 R32
R32 G22
G52 R11
R11
62 K33
K33 R23
R53 R12
R12
63 G34
G34 S24
S54 D13
D13
64 F35 F35
Y25 Y55
F14 F14
65 S36
S36 A26
A56 L15
L15
66 Y37 Y37
Y27 Y57
Y16 Y16
67 L38
L38 F28
F58 Y17
H17
68 V39
V39 M29
M59 A18
A18
69 T40
T40 V30
V60 T19 T19
70 A41 A41
G31 G61
A20 A20
71 T42
T42 A32
A62 G21
A21
72 T43
A43 M33
M63 A22
T22
73 T44
C44 G34
G64 G23
G23
74 V45
V45 L35
L65 A24
V24
75 G46
A46 L36
L66 V25
V25
76 V47
T47 S37
S67 A26
V26
77 A48
A48 S38
S68 T27
T27
78 Y49
Y49 A39
A69 G28
G28
79 A50 A50
G40 G70
A29 A29
80 A51 A51
A41 A71
A30 A30
81 K52
K52 K42
K72 --
--
82 N53
N53 S43
S73 --
--
83 V54
V54 T44
T74 --
--
84 V55 V55
V45 V75
V31 V31
85 S56
T56 E46
E76 W32
W32
86 Q57
Q57 T47
T77 P33
P33
87 F58
F58 F48
F78 L34
L34
88 V59 I59
I49 I79
I35 I35
89 S60
S60 S50
S80 N36
N36
90 S61
S61 S51
S81 Q37
Q37
91 M62 L62
M52 M82
M38 M38
92 S63
S63 T53
T83 N39
N39
93 A64 A64
A54 A84
P40 A40
94 S65 S65
T55 T85
S41 S41
95 A66 A66
A56 A86
A42 A42
96 D67 D67
D57 D87
D43 D43
97 V68 V68
V58 V88
V44 V44
98 L69
L69 L59
L89 Q45 K45
99 A70 A70
A60 A90
A46 A46
BOS CHICK Yeast
Y.Pre Rb.s Rb.c
100 M71
L71 M61
M91 L47
M47
101 S72
S72 A62
A92 A48
A48
102 K73
K73 K63
K93 S49
S49
103 I74
I74 V64
V94 I50
I50
104 E75
E75 E65
E95 F51
F51
105 I76
I76 V66
V96 V52
V52
106 K77
K77 N67
N97 D53
D53
107 L78
L78 L68
L98 V54
V54
108 S79
S79 A69
A99 S55
S55
109 D80
D80 A70
A100 S56
A56
110 I81
I81 I71
I101 V57
V57
111 P82
P82 P72
P102 E58
E58
112 E83
E83 L73
L103 P59
V59
113 G84 G84
G74 G104
G60 G60
114 K85
K85 K75
K105 V61
T61
115 N86
N86 N76
N106 Q62
Q62
116 M87
V87 V77
V107 L63
L63
117 A88
A88 V78
V108 T64
T64
118 F89
F89 V79
V109 V65
V65
119 K90
K90 K80
K110 K66
K66
120 W91
W91 W81
W111 F67
W67
121 R92
R92 Q82
Q112 L68
R68
122 G93 G93
G83 G113
G69 G69
123 K94 K94
K84 K114
K70 K70
124 P95 P95
P85 P115
P71 P71
125 L96
L96 V86
V116 I72
V72
126 F97 F97
F87 F117
F73 F73
127 V98
V98 I88
I118 I74 I74
128 R99 R99
R89 R119
R75 R75
129 H100 H100 H90 H120
R76 R76
130 R101 R101 R91 R121 R77
R77
131 T102 T102 T92 T122 T78
D78
132 K103 Q103 P93 P123
E79 E79
133 K104 A104 H94 H124
A80 K80
134 E105 E105 E95 E125
D81 D81
135 I106 I106 I96 I126 I82
I82
136 D107 N107 Q97 Q127
E83 E83
137 Q108 Q108 E98 E128
L84 L84
138 E109 E109 A99 A129
G85 A85
139 A110 A110 N100 N130 R86 R86
140 A111 E111 S101 S131 S87 S87
141 V112 V112 V102 V132 V88
V88
142 E113 D113 D103 D133 Q89 P89
143 V114 V114 M104 M134 L90 L90
144 S115 S115 S105 S135 G91 G91
145 Q116 K116 A106 A136 Q92 A92
146 L117 L117 L107 L137 L93
L93
147 R118 R118 K108 K138 V94 R94
148 D119 D119 D109 D139 D95
D95
149 P120 P120 P110 P140 T96 T96
BOS CHICK Yeast
Y.Pre Rb.s Rb.c
150 --
-- --
-- N97
S97
151 --
-- --
-- A98
A98
152 --
-- --
-- R99
E99
153 --
-- --
-- N100
N100
154 --
-- --
-- A101
A101
155 --
-- --
-- N102
N102
156 --
-- --
-- I103 K103
157 --
-- --
-- D104 P104
158 --
-- --
-- A105
--
159 --
-- --
-- G106
G105
160 --
-- --
-- A107
A106
161 --
-- --
-- E108
E107
162 Q121 Q121 Q111 Q141 A109 A108
163 H122 H122 T112 T142 T110 T109
164 D123 D123 D113 D143 D111 D110
165 L124 L124 A114 A144 Q112 E111
166 E125 D125 D115 D145 N113 N112
167 R126 R126 R116 R146 R114 R113
168 V127 V127 V117 V147 T115 T114
169 K128 K128 K118 K148 L116 L115
170 --
-- --
-- D117
P116
171 --
-- --
-- --
A117
172 --
-- --
-- --
F118
173 --
-- --
-- --
D119
174 --
-- --
-- --
G120
175 --
-- --
-- --
T121
176 --
-- --
-- E118
N122
177 K129 K129 D119 D149 A119 T123
178 P130 P130 P120 P150 G120 G124
179 E131 E131 Q121 Q151 E121 E125
180 W132 W132 W122 W152 W122 W126
181 V133 V133 L123 L153 L123 L127
182 I134 I134 I124 I154 V124 V128
183 L135 L135 M125 M155 M125 M129
184 I136 V136 L126 L156 W126 L130
185 G137 G137 G127 G157 G127 G131
186 V138 V138 I128 I158 V128 V132
187 C139 C139 C129 C159 C129 C133 ligand
188 T140 T140 T130 T160 T130 T134 '97 mutant
189 H141 H141 H131 H161 H131 H135 ligand
190 L142 L142 L132 L162 L132 L136
'97 mutant3
191 G143 G143 G133 G163 G133 G137
192 C144 C144 C134 C164 C134 C138 disulfide
193 V145 V145 V135 V165 V135 V139
194 P146 P146 P136 P166 P136 P140
195 I147 I147 I137 I167 I137 M141
196 A148 A148 G138 G168 G138 G142
197 N149 N149 E139 E169 G139 D143
198 --
-- --
-- V140
K144
199 A150 S150 A140 A170 S141 S145
BOS CHICK Yeast
Y.Pre Rb.s Rb.c
200 G151 G151 G141 G171 G142 G146
201 D152 D152 D142 D172 D143 D147
202 F153 F153 F143 F173 F144 F148
203 G154 G154 G144 G174 G145 G149
204 G155 G155 G145 G175 G146 G150
205 Y156 Y156 W146 W176 W147 W151
206 Y157 Y157 F147 F177 F148 F152
207 C158 C158 C148 C178 C149 C153
Ligand
208 P159 P159 P149 P179 P150 P154
209 C160 C160 C150 C180 C151 C155
disulfide
210 H161 H161 H151 H181 H152 H156 Ligand
211 G162 G162 G152 G182 G153 G157
212 S163 S163 S153 S183 S154 S158
213 H164 H164 H154 H184 H155 H159
214 Y165 Y165 Y155 Y185 Y156 Y160
215 D166 D166 D156 D186 D157 D161
216 A167 A167 I157 I187 S158 S162
217 S168 S168 S158 S188 A159 A163
218 G169 G169 G159 G189 G160 G164
219 R170 R170 R160 R190 R161 R165
220 I171 I171 I161 I191 I162 I166
221 R172 R172 R162 R192 R163 R167
222 K173 K173 K163 K193 K164 K168
223 G174 G174 G164 G194 G165 G169
224 P175 P175 P165 P195 P166 P170 cis-pro in
b6f, Int posn?
225 A176 A176 A166 A196 A167 A171
226 P177 P177 P167 P197 P168 P172
227 L178 Y178 L168 L198 E169 R173
228 N179 N179 N169 N199 N170 N174
229 L180 L180 L170 L200 L171 L175
230 E181 E181 E171 E201 P172 D176
231 V182 V182 I172 I202 I173 I177
232 P183 P183 P173 P203 P174 P178
233 S184 T184 A174 A204 L175 V179
234 Y185 Y185 Y175 Y205 A176 A180
235 E186 Q186 E176 E206 K177 A181
236 F187 F187 F177 F207 F178 F182
237 T188 V188 D178 D208 I179 V183
238 S189 G189 G179 G209 D180 D184
239 D190 D190 D180 D210 E181 E185
240 D191 D191 -- -- T182
T186
241 M192 L192 K181 K211 T183 T187
242 V193 V193 V182 V212 I184 I188
243 I194 V194 I183 I213 Q185 K189
244 V195 V195 V184 V214 L186 L190
245 G196 G196 G185 G215 G187 G191
>Bostaurus
SHTDIKVPDFSDYRRPEVLDSTKSSKESSEARKGFSYLVTATTTVGV
AYAAKNVVSQFVSSMSASADVLAMSKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTKKEID
QEAAVEVSQLRDP------------QHDLERVK-------KPEWVILIGVCTHLGCVPIAN
-AGDFGGYYCPCHGSHYDASGRIRKGPAPLNLEVPSYEFTSDDMVIVG
>Chicken
VHNDVTVPDFSAYRREDVMDATTSSQTSSEDRKGFSYLVTATACVAT
AYAAKNVVTQFISSLSASADVLALSKIEIKLSDIPEGKNVAFKWRGKPLFVRHRTQAEIN
QEAEVDVSKLRDP------------QHDLDRVK-------KPEWVILVGVCTHLGCVPIAN
-SGDFGGYYCPCHGSHYDASGRIRKGPAPYNLEVPTYQFVGDDLVVVG
>Sachcere
-KSTYRTPNFDDVLK---------ENNDADKGRSYAYFMVGAMGLLS
SAGAKSTVETFISSMTATADVLAMAKVEVNLAAIPLGKNVVVKWQGKPVFIRHRTPHEIQ
EANSVDMSALKDP------------QTDADRVK-------DPQWLIMLGICTHLGCVPIGE
-AGDFGGWFCPCHGSHYDISGRIRKGPAPLNLEIPAYEFDGD-KVIVG
>Rbsphaer
---------------------MSNAEDHAGTRRDFLYYATAGAGAVA
TGAA---VWPLINQMNPSADVQALASIFVDVSSVEPGVQLTVKFLGKPIFIRRRTEADIE
LGRSVQLGQLVDTNARNANIDAGAEATDQNRTLD-----EAGEWLVMWGVCTHLGCVPIGG
VSGDFGGWFCPCHGSHYDSAGRIRKGPAPENLPIPLAKFIDETTIQLG
>Rbcasul
---------------------MSHAEDNAGTRRDFLYHATAATGVVV
TGAA---VWPLINQMNASADVKAMASIFVDVSAVEVGTQLTVKWRGKPVFIRRRDEKDIE
LARSVPLGALRDTSAENANK-PGAEATDENRTLPAFDGTNTGEWLVMLGVCTHLGCVPMGD
KSGDFGGWFCPCHGSHYDSAGRIRKGPAPRNLDIPVAAFVDETTIKLG