Cytochrome c1 

Gallus gallus: NCBI BI390492 in frame 1, BBSRC 337515.1 in frame 3 

Bos taurus 

Saccharomyces cerevisiae 

Rb.capsulatus 
 

Two alternative alignmets for 50-80 region- structure suports first, less parsimonious alignment:
This adds three residues in turn at tip of 1st branch of Y-loop, removes 3 from second branch.
P74 is cis-proline. interface w sym-related c1 extends P74 - G78 (in vertebrates)
                 0        10        20        30        40        50           60        70       80
                 |         |         |         |         |         |            |         |        |
Gall2 PWSHGGPLSALDHSSVRRGFQVYKQVCSACHSMDYVAFRNLI--G-VTHTEAEAKALAEEVEVQDGPDENGEL
Gallus            GELELHPPAFPWSHGGPLSALDHSSVRRGFQVYKQVCSACHSMDYVAFRNLI--G-VTHTEAEAKALAEEVEVQDGPDENGEL
Bostaurus         SDLELHPPSYPWSHRGLLSSLDHTSIRRGFQVYKQVCSSCHSMDYVAYRHLV--G-VCYTEDEAKALAEEVEVQDGPNEDGEM
Saccharocere   MTAAEHGLHAPAYAWSHNGPFETFDHASIRRGYQVYREVCAACHSLDRVAWRTLV--G-VSHTNEEVRNMAEEFEYDDEPDEQGNP
Rb.capsulatus        NSNVQDHAFSFEGIFGKFDQAQLRRGFQVYSEVCSTCHGMKFVPIRTLSDDGGPQLDPTFVREYAAGLD--TIIDKDS-G
Rb. sphaeroides        HVEDVPFSFEGPFGTFDQHQLQRGLQVYTEVCAACHGMKFVPIRSLSEPGGPELPEDQVRAYATQFT---VTDEET-G
                                                        HHHHHHHHHHHHHHHH

                        90       100       110       120                        130        140
                         |         |         |         |                          |          |
Gall2 FMRPGKISDYFPKPYPNPEAARAANNGALPPDLSYIVNARH-----------------GGEDYVFSLLTGYC-DPPAGVVVR
Gallus          FMRPGKISDYFPKPYPNPEAARADNNGALPPDLSYIVYARH                 XXEDYVFSLLXXYX-XPPRALWC--
Bostaurus       FMRPGKLSDYFPKPYPNPEAARAANNGALPPDLSYIVRARH-----------------GGEDYVFSLLTGYC-EPPTGVSLR-
Saccharocere    KKRPGKLSDYIPGPYPNEQAARAANQGALPPDLSLIVKARH-----------------GGCDYIFSLLTGYPDEPPAGVALP-
Rb.capsulatus   EERDRKETDMFPTRVG----------DGMGPDLSVMAKARAGFSGPAGSGMNQLFKGIGGPEYIYRYVTGFPEENPACAPEG-
Rb. sphaeroides EDREGKPTDHFPHSAL-----------ENAPDLSLMAKARAGFHGPMGTGISQLFNGIGGPEYIYSVLTGFPEEPPKCAEGHE
secondary                       HH2'HH                                   HHHH3HHHHH

                    150                         160       170       180       190       200       210
                      |                           |         |         |         |         |         |
Gall2 -EGLHYNPYFP------------------GQAIGMAPPIYNEILEYDDGTPATMSQIAKDVCTFLRWAAEPEHDQRKRMGLKMLLISALLT
Bostaurus       -EGLYFNPYFP------------------GQAIGMAPPIYNEVLEFDDGTPATMSQVAKDVCTFLRWAAEPEHDHRKRMGLKMLLMMGLLL
Saccharocere    -PGSNYNPYFP------------------GGSIAMARVLFDDMVEYEDGTPATTSQMAKDVTTFLNWCAEPEHDERKRLGLKTVIILSSLY
Rb.capsulatus   IDGYYYNEVFQVGGVPDTCKDAAGIKTTHGSWAQMPPALFDDLVTYEDGTPATVDQMGQDVASFLMWAAEPKLVARKQMGLVAVVMLGLLS
Rb. sphaeroides PDGFYYNRAFQNGSVPDTCKDANGVKTTAGSWIAMPPPLMDDLVEYADGHDASVHAMAEDVSAFLMWAAEPKLMARKQAGFTAVMFLTVLS
secondary          BBBB                       BBBB                  HHHHHHHHH5HHHHHHHH HHHHHHHHHHHHH6HHHHHH

                220       230       240
                  |         |         |
Gall2 SLLYYMKRHKWSVLKSRKMAYRPPK
Bostaurus       PLVYAMKRHKWSVLKSRKLAYRPPK---
Saccharocere    LLSIWVKKFKWAGIKTRKFVFNPPKPRK
Rb.capsulatus   VMLYLTNKRLWAPYKRQKA---------
Rb. sphaeroides VLLYLTNKRLWAGVKGKK
                HHHHHHHHHHHHH