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[1] D.I. Arnon, Copper Enzymes in Isolated Chloroplasts. Polyphenoloxidase in Beta Vulgaris, Plant Physiol 24 (1949) 1-15.
Chlorophylls and Carotenoids: Measurement
and Characterization by UV-VIS Spectroscopy
Hartmut K. Lichtenthaler and Claus Buschmann
Current Protocols in Food Analytical Chemistry (2001) F4.3.1-F4.3.8
[1] O.H. Lowry, C.A. Smith and D.L. Cohen, A MICROCOLORIMETRIC METHOD FOR MEASURING THE OXYGEN SATURATION OF BLOOD, J. Biol. Chem. : . 146 (1942) 519-526.
[1] H.M. Kalckar, DIFFERENTIAL SPECTROPHOTOMETRY OF PURINE COMPOUNDS BY MEANS OF SPECIFIC ENZYMES: III. STUDIES OF THE ENZYMES OF PURINE METABOLISM, J. Biol. Chem. 167 (1947) 461-475.
[1] O. Warburg and W. Christian, Isolierung und Kristallisation des Garungsferments Enolase, Biochem. Zeitschrift 310 (1942) 384-421.
[1] J.N. Williams, Jr., A Method for the Simultaneous Quantitative Estimation of Cytochromes a, b, c1, and c in Mitochondria, Arch Biochem Biophys 107 (1964) 537-43.
[1] J. Sternberg, H. Stillo and R. Schwendeman, Spectrophotometric Analysis of Multicomponent Systems Using the Least Squares Method in Matrix Form, Analytical Chemistry 32 (1960) 84-90.
[1] E.A. Berry and B.L. Trumpower, Simultaneous determination of hemes a, b, and c from pyridine hemochrome spectra, Anal Biochem 161 (1987) 1-15.
[1] K.R. Naqvi, T.H. Hassan and Y.A. Naqvi, Expeditious implementation of two new methods for analysing the pigment composition of photosynthetic specimens, Spectrochim Acta A Mol Biomol Spectrosc 60 (2004) 2783-91.
Actually O. Warburg and Christian (1942 Biochem Z. 310, 384-?) never used matrix inversion or even algebra: they made a plot of a280/a260 vs ?? and used it to correct A280.
Lowry et al. (1942) JBC 146, 519-? used algebraic solution of a 2x2 matrix to measure concentration of oxyhemoglobin and deoxyhemoglobin.
H.M. Kalckar (JBC 167, 461-475) used Lowry's equation for the protein/nucleic acid problem and derived the equation: Protein = 1.45 E280 - 0.74 E260.
(Dave Sogan's handout) Warburg&Christian give the extinction coefficients for yeast enolase and RNA:
A260 = 22.1 [RNA] + 0.514 [Protein]
A280 = 10.8 [RNA] + 0.895 [Protein]
Fitting redox difference spectra of cytochrome complexes
spectral analysis of photosynthetic pigments chlorophyll and carotenoids
spectral analysis of photosynthetic pigments chlorophyll and carotenoids
The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents . . .