Our long-term interest is in the mechanism of energy-conserving electron
transfer chains such as the mitochondrial respiratory chain and the
photosynthetic electron transport chain. In the 1980's we worked mainly on
purification and spectroscopic characterization of components of the chain.
The field was reaching a point of understanding at which more detailed
structural information was needed before much further progress could be made.
X-ray crystallography is essentially the only source of detailed structural
information for large protein complexes. Therefore recently we have been
concentrating on crystallization of electron transport chain components,
capitalizing on our previous experience with purification.
As structures are becoming available, a lot of new research possibilities are opening
up. Some of these involve further crystallography- looking at binding
sites for substrates and inhibitors, and examining the structural effects of
point mutations. Others involve in vitro spectroscopic tests of reaction
mechanisms suggested by the structure. We intend to be involved in this
research, as well as continuing to apply our crystallization expertise to new
membrane protein complexes.
Our major funding is from NIH, Awards R01DK44842 and R01GM62563.
Additional valuable support has been provided by
The France-Berkeley Fund and Human Frontiers Science Project
Structures solved by the Berry group.
Specific Research Projects
- x-ray structure of vertebrate bc1 complexes
- bacterial bc1 complexes
- Structural studies of Respiratory Complex II
- Structural studies of cyt. cbb3 cyt. oxidase
- Structural studies of cyt. f mutants
(collaboration with J. Fernandez-Velasco and Dick Malkin's Lab at UCBerkeley)
- The cytochrome bc1 complex of trypanosomes
(with Larry Simpson, UCLA and Dmitri Maslov, U.C. Riverside)
- Spectral(vis-uv) analysis of multicomponent systems.
(sourceforge.net)
- The cytochrome bc1 complex of plant mitochondria
- Crystallization of membrane proteins
- Resources for investigating cytochrome
bc1 complexes
Current Group Members
[nobody here- we are moving to SUNY Upstate Medical University in Syracuse]
Visiting Scientists
Former Group Members
- Edward A. Berry
-
- Publications
-
- e-mail: EABerry@lbl.gov
- LiShar Huang
-
- Publications
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- e-mail: LSHuang@lbl.gov
- Ning G. Pon
-
- A more recent photo
-
- Publications
-
- e-mail: NGPon@lbl.gov
- Karen Chiang
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- Publications
-
- Undergrad Thesis: The Mechanism behind Antimycin Induced Oxidation and Heterogenous Titration
of Cytochrome bH of the Cytochrome bc1 Complex
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- e-mail: KChiang@lbl.gov
- Sheng-wen (Wendy) Tseng
-
- Publications
-
- Undergrad Thesis: The extinction coefficient of Antimycin
and an update
and later measurements by Jamie Jordan in Syracuse
-
- e-mail: SWTseng@lbl.gov
- Serena Way
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- Publications
-
- Undergrad Thesis: Cyt c552 of Vibrio cholerae
-
- small correction to thesis Cyt c552 of Vibrio cholerae
-
- e-mail: SWay@lbl.gov
- Andy Chung-Jen Wang
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- Publications
-
- e-mail: CWang@lbl.gov
- John T. Shen
-
- Publications
-
- e-mail: JTShen@lbl.gov
- Lai Saechao
-
- Publications
-
- e-mail: LKSaechao@lbl.gov
- Eric Y. Tung
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- Publications
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- e-mail: EYTung@lbl.gov
- Elise Huang
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- Publications
-
- Undergrad Thesis
-
- e-mail: EHuang@lbl.gov
- David Cobessi Former Post-Doctoral Fellow, now at CNRS-Strassbourg
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- Publications
-
- e-mail: DCobessi@lbl.gov
-
- current e-mail: David.COBESSI@esbs.u-strasbg.fr
- Thomas H Cromartie visiting from
crop protection
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- Publications
-
- e-mail: THCromartie@lbl.gov
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- current e-mail: tcromartie@byotix.com
- Matt Ban
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- Publications
-
- e-mail: MBan@lbl.gov
- Yusef Collins
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- Publications
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- e-mail: YOCollins@lbl.gov
- Zhaolei Zhang
- Web Page
- An Chian Chen
- Sequencing Rieske.
For an update on chicken cyt. bc1 sequences, see this.
- Sylvia Lo
- Sequencing cyt. c1 (I)
- Thomas Linden and Rainer Breitli
- Purification and crystallization of Cyt f and its redox partners
- Eli Kintisch
- Isolating and identifying chicken bc1 subunits
- Kyle Kwok
- Sequencing cyt c1 (II)
- Amy Fang
- Membrane protein purification and enzymology
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MAPS
- How to find our new lab in Building 64
- LBNL Building-Locator Campus Map
- Our old place in Calvin LAB on the UCCB campus
- U.C.Berkeley zoomable map and building locator
- Berkeley Hotel Map
Useful Links
- Biosci Bookmarks
- Crystallography Bookmarks
- Directory & reference Bookmarks
- User pages at UCBerkeley/LBNL Structural Biology.
- User pages at SSRL Protein crystallography beamlines.
Our favorite subject is the cytochrome bc1 complex:
Return to Structural Biology Division Home Page
Return to LBNL homepage.
Page Owner Information:
If you have questions and comments, please contact,
preferably via e-mail
Edward A. Berry,
e-mail: EABerry@lbl.gov
Lawrence Berkeley National Laboratory
MailStop 64-243
1 Cyclotron Road
Berkeley, CA 94720-8118
Phone: +1-510-486-4335
Fax: +1-510-486-6059
JFax: +1-530-323-9836 (you send fax, I receive email picture)
Home fax: +1-510-883 7803 (for non-work-related)
